Abstract
IN the liver cells of the partially hepatectomized rat and in kidney cells cultured directly from the rabbit, the stimuli to cell multiplication are not followed immediately by DNA synthesis. Instead, there is a long presynthetic period (12–18 h in rat liver1, 30 or more hours for the cultured cells2) during which metabolic changes occur that endow the cells with the ability to replicate DNA. In both the liver3 and kidney cells4 a marked increase in DNA polymerase (EC 2.7.7.7) activity occurs, beginning at about the same time as DNA replication. This temporal coincidence could mean that DNA synthesis begins after the rise in level of enzymes involved in its synthesis or, conversely, that initiation of DNA synthesis indirectly stimulates the manufacture of higher levels of enzymes and substrates needed for replication.
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ADAMS, R., ABRAMS, R. & LIEBERMAN, I. Rise in Deoxyribonucleic Acid Polymerase Activity in the Absence of Deoxyribonucleic Acid Synthesis in Cultured Kidney Cells. Nature 206, 512–513 (1965). https://doi.org/10.1038/206512a0
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DOI: https://doi.org/10.1038/206512a0
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