Abstract
A CONSIDERABLE body of evidence now supports the proposal of Orloff and Handler1 that adenosine-3′,5′-monophosphate (cyclic AMP) is an intracellular mediator of the antidiuretic response to the neurohypophyseal nonapeptides. Experiments with an isolated target tissue, the urinary bladder of the toad, showed that the permeability changes occur at the urinary (mucosal) surface of the responsive cell; the nonapeptides, however, are active only via the body fluid (serosal) surface2. Knowledge of the cellular level at which the nonapeptides react with initial receptors is therefore valuable in defining the mode of the hormonal action. Recent studies on the uptake of 3H-oxytocin by the toad bladder showed rapid and continual penetration of the hormone into the cells3. The local conditions (ionic composition, pH, etc.) vital to the initial hormonal interaction, therefore, may well be those within the cell.
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GULYASSY, P. Intracellular H+ Concentration of the Isolated Urinary Bladder of the Toad. Nature 206, 511–512 (1965). https://doi.org/10.1038/206511a0
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DOI: https://doi.org/10.1038/206511a0
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