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Electrophoresis of 35S-labelled Material released from Clumping Platelets

Nature volume 205, pages 711–712 (1965)Cite this article

Abstract

RAT platelets contain a sulphated mucopolysaccharide1 which, when subjected to paper electrophoresis and to paper chromatography, showed a mobility similar to chondroitin sulphate-A but unlike that of heparin1,2. The acid mucopolysaccharide (AMPS) content of human serum increased during the early phase of the blood clotting process3; thrombocytes were suggested as a possible source of this additional serum mucopolysaccharide. When thrombin was added in vitro to a suspension of 35S-labelled pig platelets, up to 50 per cent of the radioactivity was lost from the platelets during clumping4. Electrophoretic identification of this released material has been attempted in the present investigation.

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References

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  2. Odell, jun., T. T., and Anderson, B., Proc. Soc. Exp. Biol. and Med., 94 (1957).

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  4. Geissinger, H. D., Mustard, J. F., Riddell, P. E., Robinson, G. A., and Rowsell, H. C., Brit. J. Haem., 9 (1963).

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Authors and Affiliations

  1. Department of Physiological Sciences, Ontario Veterinary College, Guelph, Ontario, Canada

    PATRICIA E. RIDDELL & A. M. BIER

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  1. PATRICIA E. RIDDELL
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  2. A. M. BIER
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RIDDELL, P., BIER, A. Electrophoresis of 35S-labelled Material released from Clumping Platelets. Nature 205, 711–712 (1965). https://doi.org/10.1038/205711a0

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