Abstract
THE zone electrophoresis of human parotid saliva proteins has been carried out with a variety of supporting media, including filter paper, cellulose acetate, agar gel and starch gel, with separations usually of from five to twelve fractions1–8. In a comparative investigation of these media, D'Silva and Ferguson7 obtained their most satisfactory results with a combination of cellulose acetate and starch gel, thereby separating seventeen protein components from the parotid saliva of one subject. The present report concerns an examination of acrylamide gel as another medium for the zone electrophoresis of parotid saliva proteins. Preliminary experiments with acrylamide-gel strips have yielded protein patterns in which twenty or more fractions could be visually distinguished when the electrophoresis was carried out in 0.1 M tris-EDTA–boric acid buffer at pH. 9.0. The tests were conducted on individual collections of parotid saliva from three subjects and on pooled collections of parotid saliva from several subjects. Fig. 1 shows the electrophoresis pattern of a sample of the pooled parotid saliva. Under the conditions of the experiment, more than half the staining components migrated anodically with generally better resolution than is evident for the cathodically-migrating components. Although disk electrophoresis on acrylamide gel has been widely used in investigations of serum proteins, gel strips were used for this investigation of parotid saliva in order to obtain the complete pattern of staining components, migrating both anodically and cathodically, for each electrophoresis run. Gel strips also permit the handling of larger quantities of protein than is feasible with disk electrophoresis, should recovery of particular fractions be desired. Salivary amylase, a troublesome factor in the starch-gel electrophoresis of saliva proteins, does not attack acrylamide gel.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Drevon, B., and Donikian, R., C.R. Soc. Biol., 150, 1206 (1956).
Köstlin, A., and Rauch, S., Helv. Med. Acta, 24, 600 (1957).
Ferguson, M. H., Krahn, H. P., and Hildes, J. A., Canad. J. Biochem. Physiol., 36, 1001 (1958).
Weinstein, E., Heskins, D., and Jackson, S. W., J. Dent. Res., 39, 110 (1960).
Mandel, I. D., and Ellison, S. A., Arch. Oral Biol., 3, 77 (1961).
Holen, S., Haber, N., and Tuck-Weiss, I., J. Dent. Res., 40, 470 (1961).
D'Silva, J. L., and Ferguson, D. B., Arch. Oral Biol., 7, 563 (1962).
Hoerman, K. C., J. Lab. Clin. Med., 53, 64 (1959).
Raymond, S., and Wang, Y., Anal. Biochem., 1, 391 (1960); Raymond, S., and Nakamichi, M., Anal. Biochem., 3, 23 (1962).
Ornstein, L., and Davis, B. J., Disc Electrophoresis (Distillation Products Industries, 1961).
Curby, W. A., J. Lab. Clin. Med., 41, 493 (1953).
Ferris, T. G., Easterling, R. E., and Budd, R. E., Amer. J. Clin. Path., 38, 383 (1962).
Ellison, S. A., Mashimo, P. A., and Mandel, I. D., J. Dent. Res., 39, 892 (1960).
Stoffer, H. R., Kraus, F. W., and Holmes, A. C., Proc. Soc. Exp. Biol. and Med., 111, 467 (1962).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MEYER, T., LAMBERTS, B. Zone Electrophoresis of Human Parotid Saliva in Acrylamide Gel. Nature 205, 1215–1216 (1965). https://doi.org/10.1038/2051215a0
Published:
Issue Date:
DOI: https://doi.org/10.1038/2051215a0
This article is cited by
-
Invertase in cell-free culture fluids ofstreptococcus mutans strain SL-1
Experientia (1975)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.