Abstract
CRITICAL analysis of methods for assaying lengths of fungal mycelium present in unit weights or volumes of soil1 has shown that the agar film technique2,3 is least prone to experimental error. The technique has been used successfully in these laboratories for a number of years, and a number of slight modifications have proved useful in eliminating accidental errors and increasing the reliability of the technique. These modifications are incorporated in the following description of the procedure.
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References
Nicholas, D. P., Ph.D. thesis, University of Liverpool (1962).
Jones, P. C. T., and Mollison, J. E., J. Gen. Microbiol., 2, 54 (1948).
Skinner, F. A., Jones, P. C. T., and Mollison, J. E., J. Gen. Microbiol., 6, 261 (1952).
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THOMAS, A., NICHOLAS, D. & PARKINSON, D. Modifications of the Agar Film Technique for assaying Lengths of Mycelium in Soil. Nature 205, 105 (1965). https://doi.org/10.1038/205105a0
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DOI: https://doi.org/10.1038/205105a0
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