Abstract
METHODS of obtaining kidney glomeruli for the determination of enzyme content are microdissection of cryostat sections or differential centrifugation of kidney homogenates in sucrose. We wish to report alkaline phosphatase determinations on glomeruli dissected from fragments of frozen-dried rat kidneys. This method of preparation has the practical advantage of being simple and convenient, and may also avoid enzyme destruction by freeze-thawing or solution in aqueous media. Preliminary observations on the effect of nephrotoxic serum are also reported.
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O'BRIEN, M., CHASE, W. Quantitative Enzyme Histochemistry using Frozen-dried Glomeruli. Nature 202, 304–305 (1964). https://doi.org/10.1038/202304a0
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DOI: https://doi.org/10.1038/202304a0
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