Abstract
PHENOLPHTHALEIN diphosphate was introduced by King1 as a substrate for phosphatases. Huggins and Talalay2 applied it to quantitative determinations of enzyme activities. They showed that the amount of phenolphthalein liberated by soluble phosphatases of animal origin increases more than linearly with the time of reaction. The same relation is found when varying the concentration of the enzyme instead of the time of reaction.
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References
King, E. J., J. Path. and Bact., 55, 311 (1943).
Huggins, C., and Talalay, P., J. Biol. Chem., 159, 399 (1945).
Schmidt, G., Hecht, L., and Thannhauser, S., J. Biol. Chem., 178, 733 (1949).
Suomalainen, H., Linko, M., and Oura, E., Biochim. Biophys. Acta, 37, 482 (1960).
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PAUWELS, G. Occurrence of Phenolphthalein Monophosphate as an Intermediate in the Enzymatic Hydrolysis of Phenolphthalein Diphosphate to Phenolphthalein and Orthophosphate. Nature 202, 190–192 (1964). https://doi.org/10.1038/202190a0
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DOI: https://doi.org/10.1038/202190a0
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