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Immunoassay of Growth Hormone in Cultures of Human Hypophysis by the Method of Complement Fixation: Comparison of the Growth Hormone Secretion and the Prolactin Activity

Abstract

SINCE the isolation of purified human growth hormone (H.G.H.), immunoassays of this hormone, with variable results regarding sensitivity, accuracy and reproducibility, have been reported1–3. A new approach to the immunoassay of minute amounts of human growth hormone in biological fluids was attempted by way of the well-known complement-binding method; it proved to be relatively simple, reliable and quantitatively satisfactory.

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BRAUMAN, J., BRAUMAN, H. & PASTEELS, J. Immunoassay of Growth Hormone in Cultures of Human Hypophysis by the Method of Complement Fixation: Comparison of the Growth Hormone Secretion and the Prolactin Activity. Nature 202, 1116–1118 (1964). https://doi.org/10.1038/2021116a0

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