Abstract
IN the course of an investigation concerning lens RNA's and their role in the biosynthesis of proteins and ageing1–5. we have examined soluble RNA and particularly its ability to bind amino-acids. In 1961 Devi and Lerman6,7 investigated the incorporation of amino-acids labelled with carbon-14 into lens-protein and RNA. The authors have measured the total incorporation of amino-acids into protein and, on the other hand, into the lens RNA proteins complex. The difference between these two values was taken to indicate the activity of lens RNA fraction alone. It should be noted that these experiments were performed with a homogenate containing the different types of RNA and that the RNA protein complex also contained types of RNA other than soluble RNA.
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References
Mandel, P., Klethi, J., Virmaux, N., and Lessinger, A., C.R. Acad. Sci., Paris, 248, 476 (1959).
Virmaux, N., Klethi, J., and Mandel, P., C.R. Soc. Biol., 152, 1570 (1958).
Virmaux, N., and Mandel, P., C.R. Soc. Biol. Paris, 155, 620 (1961).
Mandel, P., Virmaux, N., and Klethi, J., C.R. Soc. Biol., 156, 2123 (1962).
Virmaux, N., Klethi, J., and Mandel, P., J. Physiol. (Paris), 55, 356 (1963).
Devi, A., Friel, R., and Lerman, S., Arch. Ophthal., 65, 855 (1961).
Devi, A., Lerman, S., and Sarkar, N. K., Nature, 190, 1193 (1961).
Hoagland, M. B., in The Nucleic Acids, edit. by Chargaff, E., and Davidson, J. N., 3, 349 (Academic Press, New York, 1960).
Hoagland, M. B., Stephenson, M. L., Scott, J. F., Hecht, L. I., and Zamecnik, P. C., J. Biol. Chem., 231, 241 (1958).
Preiss, J., Berg, P., Ofengand, E. J., Bergmann, F. H., and Dieckmann, M., Proc. U.S. Nat. Acad. Sci., 45, 319 (1959).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).
Allen, E. H., Glassmann, E., Schweet, R. S., J. Biol. Chem., 235, 1061 (1960).
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VIRMAUX, N., MANDEL, P. Evidence of an Aminoacyl Ribonucleic Acid Synthetase Activity in Calf Lens. Nature 201, 297–298 (1964). https://doi.org/10.1038/201297a0
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DOI: https://doi.org/10.1038/201297a0
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