Abstract
IMMUNE lysis of tissue cells is more difficult to measure than immune hæmolysis because the material lost by lysing cells is not coloured, as hæmoglobin is. Greon, Fleischer, Barrow and Goldberg1 among others have shown that during immune lysis of ascites tumour cells, cytoplasmic nucleic acid is released from the cells, mainly as nucleoprotein. We have found it practicable to use this to measure cell lysis, and it is much easier than direct microscopic examination of the cells for measurement of damage2. Using this method it is possible to titrate both complement and cytolytic antibody against guinea pig peritoneal exudate cells (chiefly mononuclear cells) and Ehrlich ascites tumour cells with little more difficulty than comparable titrations using hæmolysin and red cells involve.
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References
Green, H., Fleischer, R. A., Barrow, P., and Goldberg, B., J. Exp. Med., 109, 511 (1959).
Reif, A. E., J. Immunol., 89, 849 (1962).
Ogur, M., and Rosen, G., Arch. Biochem., 25, 262 (1950).
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ROMEYN, J., RIGBY, C. Measurement of Immune Cytolysis. Nature 201, 1142–1143 (1964). https://doi.org/10.1038/2011142a0
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DOI: https://doi.org/10.1038/2011142a0
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