Abstract
A METHOD was recently developed in this laboratory by which it was possible to degrade DNA chemically to sequences of purine nucleotides1,2. During this procedure the DNA is first freed of pyrimidines by heating with anhydrous hydrazine and then degraded in alkali; the fragments formed are separated by chromatography on DEAE cellulose in a lithium chloride gradient. Accurate quantitative analysis revealed that most purine nucleotides occur in calf-thymus DNA in sequences of two or more purine nucleotides3. The results make it possible to calculate the frequency of occurrence of the individual purine nucleotide sequences in the DNA molecule (Table 1).
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References
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HABERMANN, V. Participation of Purine and Pyrimidine Nucleotide Sequences in the Molecule of Calf Thymus Deoxyribonucleic Acid. Nature 200, 782–783 (1963). https://doi.org/10.1038/200782a0
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DOI: https://doi.org/10.1038/200782a0
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