Abstract
THE disassociation of cells from various parent tissues by digestion with trypsin has been often applied by workers on tissue cultures1–3. There are, however, several defects in the trypsinization method when the goal is use of the cells for physiological investigations. We have examined various possible methods for avoiding these difficulties and have found that the substitution of collagenase for trypsin satisfied most of our requirements.
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References
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CAVANAUGH, D., BERNDT, W. & SMITH, T. Disassociation of Heart Cells by Collagenase. Nature 200, 261–262 (1963). https://doi.org/10.1038/200261b0
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DOI: https://doi.org/10.1038/200261b0
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