Embryos derived from Callus Tissue Cultures of the Wild Carrot

Abstract

INDEPENDENT regeneration of root or shoot primordia is common in higher plants. However, the simultaneous, integrated development of both primordia from a single small mass of meristematic cells is found almost exclusively in ovular embryos. We have observed the development of structures bearing both these primordia, from callus tissue cultures of the wild carrot, Daucus carota L. These structures closely resemble in situ pro-embryos and embryos both morphologically and in their pattern of development.