Abstract
FOR the past months, we have been examining the lytic agents affecting yeast and mould cell-walls. Micro-organisms capable of lysing the cell-walls of these organisms were isolated from soil samples. The digestion of the cell-wall material of mould spores, by means of a partially purified enzyme produced by the Streptomyces GM, has been described already in a previous communication1. Some of us, using the method of digestion by the strepzyme or the gastric juice of the snail Helix pomatia, have published several reports on their investigations with protoplasts prepared from various moulds2–5. The results obtained clearly demonstrated that protoplasts of Trichotecium roseum could be produced by using the lytic enzymes under appropriate experimental conditions.
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References
Garcia Acha, I., and Villanueva, J. R., Canad. J. Microbiol., 9, 139 (1963).
Garcia Mendoza, C., and Villanueva, J. R., Nature, 195, 1326 (1962).
Garcia Mendoza, C., and Villanueva, J. R., Microbiol. Espan., 15, 139 (1932).
R. Aguirre, M. J., and Villanueva, J. R., Nature, 196, 639 (1962).
R. Aguirre, M. J., Garcia Acha, I., and Villanueva, J. R., Experientia, 19, 82 (1963).
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GARCIA ACHA, I., VILLANUEVA, J. Differences in the Mode of Action of Strepzyme and Helix pomatia Enzyme Preparations on Trichotecium roseum Spores. Nature 200, 1231 (1963). https://doi.org/10.1038/2001231a0
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DOI: https://doi.org/10.1038/2001231a0
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