Abstract
A CHARACTERISTIC pink coloration was observed by Kohn and Liversedge1 after heating tissue suspensions with aqueous thiobarbituric acid (TBA). Colour formation was attributed to reaction of unknown oxidation products of unsaturated fatty acids2. The principal TBA-reactive substance is considered to be malonaldehyde3,4. However, the means of formation of malonaldehyde is unknown. Inasmuch as ultra-violet irradiation of unsaturated fatty acids had been used to produce TBA-reactive products5, investigation of the radiation-induced oxidation was conducted in this work in an attempt to clarify the basis of the TBA test.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Kohn, H. I., and Liversedge, M., J. Pharmacol. Exp. Therap., 82, 292 (1944).
Bernheim, F., Bernheim, M. L. C., and Wilbur, K. M., J. Biol. Chem., 174, 257 (1948).
Patton, S., and Kurtz, G. W., J. Dairy Sci., 34, 669 (1951).
Patton, S., Keeney, M., and Kurtz, G. W., J. Amer. Oil Chem. Soc., 28, 391 (1951).
Kenaston, C. B., Wilbur, K. M., Ottolenghi, A., and Bernheim, F., J. Amer. Oil Chem. Soc., 32, 33 (1955).
Waravdekar, V. S., and Saslaw, L. D., Biochim. Biophys. Acta, 24, 439 (1957).
Waravdekar, V. S., and Saslaw, L. D., J. Biol. Chem., 234, 1945 (1959).
Saslaw, L. D., and Waravdekar, V. S., J. Org. Chem., 22, 843 (1957).
Saslaw, L. D., Anderson, H. J., and Waravdekar, V. S., Fed. Proc., 22, 199 (1963).
Claisen, L., Ber., 36, 3664 (1903).
Fränz, J., and Cole, B. T., Arch. Biochem. Biophys., 96, 382 (1962).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SASLAW, L., ANDERSON, H. & WARAVDEKAR, V. Ultra-violet Photolysis of Unsaturated Fatty Acids in Relation to the Thiobarbituric Acid Test. Nature 200, 1098–1099 (1963). https://doi.org/10.1038/2001098a0
Issue Date:
DOI: https://doi.org/10.1038/2001098a0
This article is cited by
-
Action of UV radiation on human blood serum under normal and pathological conditions
Bulletin of Experimental Biology and Medicine (1977)
-
Reliability of the Thiobarbituric Acid Test in the Presence of Inorganic Iron
Nature (1966)
-
Malonaldehyde from the Autoxidation of Methyl Linolenate
Nature (1966)
-
Infra-red Spectra and Chromatographic Fractions of Irradiated Linoleic and Linolenic Acids
Nature (1965)
-
Hepatotropic Effect in Mice by an Aqueous Extract of Ultra-violet-irradiated Linolenic Acid
Nature (1964)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
