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Separation of Iron-containing Ferritin from Horse-spleen into Three Distinct Fractions by Starch-gel Electrophoresis

Nature volume 198, pages 892–893 (1963)Cite this article

Abstract

ULTRACENTRIFUGAL investigations1 have shown that ferritin which was prepared from horse-spleen by Granick's method2 is not a chemical entity but consists of a mixture of a colourless component and a heterogeneous coloured material. The colourless component is an iron-free, fairly homogeneous protein (S20= 17.6;mol. wt. 465,000), moving with a sharp boundary in the ultracentrifuge, and is identical with apoferritin prepared by Granick and Michaelis3. The coloured iron-containing material, ferritin, is rather heterogeneous and sediments with a widely spread boundary.

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References

  1. Rothen, A., J. Biol. Chem., 152, 679 (1944).

    CAS  Google Scholar 

  2. Granick, S., J. Biol. Chem., 146, 451 (1942).

    CAS  Google Scholar 

  3. Granick, S., and Michaelis, L., J. Biol. Chem., 147, 91 (1943).

    CAS  Google Scholar 

  4. Mazur, A., Litt, I., and Shorr, E., J. Biol. Chem., 187, 473 (1950).

    PubMed  CAS  Google Scholar 

  5. Mazur, A., Baer, S., and Shorr, E., J. Biol. Chem., 213, 147 (1955).

    PubMed  CAS  Google Scholar 

  6. de Bornier, B. M., Bull. Soc. Chim. Biol., 39, 1067 (1957).

    PubMed  CAS  Google Scholar 

  7. Smithies, O., Biochem. J., 61, 629 (1955).

    Article  CAS  Google Scholar 

  8. Smithies, O., Biochem. J., 71, 585 (1959).

    Article  CAS  Google Scholar 

  9. Hughes, jun., W. L., J. Amer. Chem. Soc., 69, 1836 (1947).

    Article  CAS  Google Scholar 

  10. Hughes, jun., W. L., Cold Spring Harbor Symp. Quant. Biol., 14, 79 (1949).

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Hygiene-Institut der Universität, Freiburg/Br., Germany

    RUDOLF KOPP, ARNOLD VOGT & GUNTER MAASS

Authors
  1. RUDOLF KOPP
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  2. ARNOLD VOGT
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  3. GUNTER MAASS
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KOPP, R., VOGT, A. & MAASS, G. Separation of Iron-containing Ferritin from Horse-spleen into Three Distinct Fractions by Starch-gel Electrophoresis. Nature 198, 892–893 (1963). https://doi.org/10.1038/198892a0

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