Abstract
THE freeze-drying technique permits the embedding of tissue containing water-soluble radioactively labelled compounds with minimal movement of the labelled material. However, the procedure for mounting the microtome sections later obtained from the tissue usually requires a mounting adhesive containing water or actually floating the ribbon of sections on water. During the latter procedure large amounts of radioactivity are lost from the sections—up to 100 per cent in some cases1. Using the ‘mounting adhesive technique’ the radioactivity may not be lost from the section; but, what is worse, it may move, giving a false impression of the previous localization in the tissue.
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SMITHERMAN, T., DEBONS, A., PITTMAN, J. et al. Movement of Water-soluble Material in Mayer's Albumin and a Simplified Dry-mounting Method for Autoradiography. Nature 198, 499–500 (1963). https://doi.org/10.1038/198499a0
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DOI: https://doi.org/10.1038/198499a0
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