Abstract
BARBITURATES isolated from biological fluids and tissues are not sufficiently pure for characterization by crystal tests. Purification and separation, if one or more barbiturates are present in the isolated product, are prerequisites for such tests. Paper chromatography and sublimation techniques have been reported in numerous publications to attain sufficient purity of barbiturates for these purposes. Sublimation technique for purification is not consistently successful on barbiturates isolated from biological specimens. A combination of paper chromatography and sublimation was successfully applied to obtain the barbiturates in pure state for characterization by crystal tests. This method was used by us on micro-quantities of barbiturates isolated from blood and other tissue materials.
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Neil, M. W., and Payton, J. E., Med. Sci. and Law, 2, 4 (Oct. 1961).
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GUPTA, R., KOFOED, J. Separation, Purification and Identification of Some Common Barbiturates in Toxicological Analyses. Nature 198, 384 (1963). https://doi.org/10.1038/198384a0
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DOI: https://doi.org/10.1038/198384a0
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