Electrophoretic Analysis of Serum and Pleuroperitoneal Fluid Proteins and Antibody from Guinea Pigs

Abstract

INTRAPERITONEAL injection of Freund's adjuvant (with or without antigen) into mice induces the production of ascitic fluid^1–5. Ascitic fluid contains γ-, β₂-, β₁- α₂-, α₁-globulin and albumin determined by moving boundary electrophoresis^4,5. Anti-bovine serum albumin precipitins in mouse ascitic fluid were identified as β₂-globulins^4,5. This work has been extended to include the guinea pig. In many of the animals ascites formation was also associated with fluid accumulation in the thoracic cavity. Serum, pleural and peritoneal fluids were characterized by moving boundary electrophoresis. Preliminary evidence indicated little or no electrophoretic difference between pleural and peritoneal fluids. Since it is possible that pleural fluid accumulates as a result of ascites formation, these fluids collectively are referred to as pleuroperitoneal fluid.