Abstract
IN earlier publications1,2 we described the preparation of soluble glucose-6-phosphatase (G6Pase) from microsomes of bovine liver. We always found that a combined treatment of the microsomes with sodium deoxycholate and digitonin leads to a reproducible solubilization of this enzyme. However, it was noted that G6Pase is very sensitive towards sodium deoxycholate and under the latter's influence activity is markedly diminished.
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References
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GÖRLICH, M., HEISE, E. Solubilization and Proteolytic Activation of Glucose-6-phosphatase from Microsomes of Bovine Livers. Nature 197, 698–699 (1963). https://doi.org/10.1038/197698a0
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DOI: https://doi.org/10.1038/197698a0
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