Abstract
DURING studies1 on the α-amylase from the hepatopancreas of Carcinus maenas, the common shore crab, the need arose for a purified enzyme preparation. Highly purified preparations of α-amylases have been prepared previously using starch as an adsorbent2,3, but partition systems consisting of starch in water have serious drawbacks for chromatographic purposes since commercial starches are of poorly defined composition4. However, by using dextran-gel columns a purification stage has been developed which is reproducible and which gives a good enrichment.
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References
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BLANDAMER, A., BEECHEY, R. Dextran-gel Filtration in the Purification of a Crustacean α-Amylase. Nature 197, 591–592 (1963). https://doi.org/10.1038/197591a0
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DOI: https://doi.org/10.1038/197591a0
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