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Effect of Bromouracil-labelled Transforming Deoxyribonucleic Acid of Bacillus subtilis on Cell Growth

Nature volume 197, pages 407–408 (1963)Cite this article

Abstract

BROMOURACIL inhibits bacterial growth through its incorporation into the deoxyribonucleic acids in place of thymine1; however, infectivity of bacteriophage2 and transforming activity3 remain even though the DNA is heavily labelled with bromouracil. In this communication we wish to report on the incorporation of bromouracil into Bacillus subtilis wild-type DNA and its effects on cell growth when the organisms are plated on the medium used for enumeration of the total cell number in transformation experience.

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References

  1. Handschumacher, R. E., and Welch, A. D., in The Nucleic Acids, edit. by Chargaff, E., and Davidson, J. N., 3, 453 (Academic Press, New York, 1960).

    Book  Google Scholar 

  2. Litman, R. M., and Pardee, A. B., Nature, 178, 529 (1956).

    Article  ADS  CAS  Google Scholar 

  3. Ephrati-Elizur, E., and Zamenhof, S., Nature, 184, 472 (1959).

    Article  ADS  CAS  Google Scholar 

  4. Szybalski, W., Opara-Kubinska, Z., Lorkiewicz, L., Ephrati-Elizur, E., and Zamenhof, S., Nature, 188, 743 (1960).

    Article  ADS  CAS  Google Scholar 

  5. Spizizen, J., Proc. U.S. Nat. Acad. Sci., 44, 1072 (1958).

    Article  ADS  CAS  Google Scholar 

  6. McDonald, W. (personal communication).

  7. Djordjevic, B., and Szybalski, W., J. Exp. Med., 112, 509 (1960).

    Article  CAS  Google Scholar 

  8. Young, F. E., and Spizizen, J., J. Bact., 81, 823 (1961).

    CAS  PubMed  Google Scholar 

  9. Szybalski, W., Opara-Kubinska, Z., and Ephrati-Elizur, E., Fed. Proc., 19, 306 (1960).

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Oklahoma State University, Stillwater

    DIXIE L. GIMLIN, EGBERT FARQUHARSON & FRANKLIN R. LEACH

Authors
  1. DIXIE L. GIMLIN
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  2. EGBERT FARQUHARSON
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  3. FRANKLIN R. LEACH
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GIMLIN, D., FARQUHARSON, E. & LEACH, F. Effect of Bromouracil-labelled Transforming Deoxyribonucleic Acid of Bacillus subtilis on Cell Growth. Nature 197, 407–408 (1963). https://doi.org/10.1038/197407a0

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  • DOI: https://doi.org/10.1038/197407a0

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