Abstract
IT is well known that the sudan and related dyes used for demonstrating lipids in histochemical technique dissolve in the lipid and can be re-extracted with fat solvents. Such oil-colorants are more soluble in oil than in the solvent used, and if an oil is shaken with a great excess of colorant solution, the oil will become saturated with colorant. Gomori1 has introduced triethyl phosphate as a solvent for oil colorants in histochemistry for it has the advantage of being miscible with water. If the colorant is dissolved in triethyl phosphate, therefore, excess colorant may be removed by addition of water without re-extraction of colorant from the oil. The amount of oil will be proportional to the amount of colorant dissolved, and this may be measured photometrically.
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References
Gomori, G., Microscopic Histochemistry (Chicago, 1952).
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DALES, R. A Colorimetric Method for the Quantitative Measurement of Total Lipids in Tissues. Nature 197, 1120–1121 (1963). https://doi.org/10.1038/1971120a0
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DOI: https://doi.org/10.1038/1971120a0
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