Abstract
THE identification of the origin of various animal proteins proved possible by the agar-gel double diffusion technique1. Antisera were prepared in rabbits by multiple injections of alum-precipitated animal sera. Antigens were prepared from animal sera and tissue extracts, tick blood-meals, sun-dried meat or biltong and uncooked sausage meat. They were diffused overnight at 25° C against known antisera simultaneously with the homologous antigen. The agar gels were prepared from 1 per cent agar in borate buffer at pH 8.6 and were about 3 mm thick. A seven-well pattern was used consisting of a 9.5-mm diameter central well set 10 mm from the 7-mm diameter peripheral antigen wells.
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Ouchterlony, O., Ark. Kemi. Mineral. Geol., B, 26, 1 (1948).
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HAY, D. Identification of the Origin of Animal Protein by Agar-gel Diffusion. Nature 196, 995 (1962). https://doi.org/10.1038/196995a0
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DOI: https://doi.org/10.1038/196995a0
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