Hæmadsorption to Single Cells in Suspension

Abstract

IN work on the reproduction of viruses there is often need to determine the number of cells reaching a certain stage in the multiplication cycle at a given time. Techniques involving fluorescent antibody, autoradiography, staining for inclusion bodies or hæmadsorption satisfactorily identify the infected cells in a monolayer culture, but the negative cells are almost impossible to count with certainty because their borders tend to be indistinct. Moreover, it is not possible to make continuous observations on a single population of cells since a culture must be killed every time a reading is made.