Abstract
SYNCYTIAL nuclei, following isolation and staining by Feulgen's reaction, form an irregular pattern consisting of clumps, with single nuclei scattered in between. It is possible to estimate the deoxyribonucleic acid (DNA) content of single syncytial nuclei by microspectrophotometry, but not those in clumps, due to overlapping and lack of definity. It was considered that proteolytic enzymes could break up the clumps, but it was not known whether they would also damage the nuclei. In this investigation, papain and trypsin were used to break up the clumps, and their effect observed on nuclear size, shape and DNA content.
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References
Quinlivan, W. L. G., Amer. J. Obstet and Gynec., 84, 8, 1065 (1962).
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QUINLIVAN, W. Deoxyribonucleic Acid Content of Syncytial Nuclei treated with Papain and Trypsin. Nature 196, 694–695 (1962). https://doi.org/10.1038/196694b0
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DOI: https://doi.org/10.1038/196694b0
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