Abstract
THE paper disk assay method is frequently used for the detection of penicillin in milk. An overnight test with the cylinder-plate method using Sarcina lutea as the test organism can detect the presence of 0.005 I.U. of penicillin/ml. of milk1. Another method using Bacillus subtilis and a thin layer (6 ml.) of seeded agar can detect 0.05 I.U./ml. within 2½–3 h (ref. 2).
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References
Anon, Tentative Methods for the Determination of Antibiotics in Milk. Revised August 1958 (Food and Drug Administration, Washington, D.C.).
Standard Methods for the Examination of Dairy Products, seventh ed., 185 (American Public Health Association, Inc., New York, 1960).
Cerny, J., and Morris, R. L. A., J. Milk and Food Tech., 18, 281 (1955).
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MEI, H., CLEGG, L. An Increase in Sensitivity for the Assay of Penicillin in Milk. Nature 196, 691–693 (1962). https://doi.org/10.1038/196691b0
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DOI: https://doi.org/10.1038/196691b0
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