Abstract
KENNEDY1 showed that the phospholipids in a rat liver homogenate, suitably fortified and buffered, became labelled in the presence of inorganic phosphorus-32. After the addition of adenosine triphosphate but not cytidine triphosphate most of the phosphorus-32 was present in an acidic fraction which appeared to be mainly phosphatidic acid but which was not fully characterized. Later work2 showed that phosphorus-32 exchange with lecithin, sphingomyelin, phosphatidyl ethanolamine and phosphatidyl inositol was dependent on cytidine triphosphate and the labelling with adenosine triphosphate alone was assumed to involve only phosphatidic acid. More recent work3 has shown that labelling with adenosine triphosphate could occur also with some inositol phospholipids.
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COLLINS, F. Labelling of Phospholipids with Phosphorus-32 in Rat Liver Homogenates. Nature 196, 671–672 (1962). https://doi.org/10.1038/196671a0
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DOI: https://doi.org/10.1038/196671a0
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