Abstract
MOST basidiomycete cultures, whether isolated from decay in wood, from portions of basidiocarps, or from spore casts, are dicaryotic. Monocaryotic mycelia needed for special investigations, such as interfertility testing, are usually obtained from single basidiospores. Monocaryons may, however, arise directly from the dicaryotic mycelium of some species during culture on artificial media, and in most species they can be produced from dicaryons by microsurgery1,2, by high-speed blending3,4, or by the action of cholic acid derivatives5.
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References
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DA COSTA, E., KERRUISH, R. Production of Monocaryons in Basidiomycete Cultures by the Action of Toxic Chemicals. Nature 195, 726–727 (1962). https://doi.org/10.1038/195726b0
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DOI: https://doi.org/10.1038/195726b0
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