Acrylamide Gel as an Electrophoresis Medium

Abstract

THE use of acrylamide gel as a supporting medium for electrophoretic separation was forecast by Ornstein¹ and by Raymond and Weintraub², and was rendered feasible by the work of Dr. S. Raymond³ of the William Pepper Laboratory, University of Pennsylvania, with Wang⁴, Nakamichi⁵, and Nakamichi, Brodsky and Bryant⁶. At present, the procedure is as follows. ‘Cyanogum 41^(R)’ monomer mixture, 5 per cent dissolved in tris-ethylenediamine tetracetic acid-borate buffer⁴ is mixed with 0.2 per cent catalyst as described by Raymond and Wang⁴ and poured into a special electrophoresis cell (Fig. 1) which was devised by Raymond and is to be described in detail elsewhere³. However, the E–C Apparatus Corporation of Swarthmore, Pennsylvania, has recently marketed the apparatus and materials for the application of this technique, which this corporation calls ‘Vertical gel electrophoresis’.