Abstract
THERE seems to be a need for a quantitative cytoohemical technique for assessment of glycogen within intact cellular structures. In histochemical detection of glycogen, only the fraction of total cellular glycogen soluble in trichloroacetic acid takes part in the staining reaction1. To overcome this difficulty, an attempt has been made to establish the present technique for measuring the absolute dry mass of glycogen of a cell. Danielli2 recorded that after fixation of the tissue in absolute alcohol mainly protein, nucleic acid and carbohydrate remain in the cellular material.
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References
Kugler, J. H., and Wilkinson, W. J. C., J. Histochem. Cytochem., 8, No. 3, 195 (1960).
Danielli, J. F., General Cytochemical Methods, 1, 72 (Academic Press, Inc., Pub., 1958).
Chatterjee, R., Bose, A., and De, P., Intern. J. Rad. Biol., 1, No. 4, 420 (1959).
Lillie, R. D., Histo-Pathologic Technic and Practical Histochemistry, 275 (The Blakiston Co., Inc., New York, 1954).
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DE, P., CHATTERJEE, B. A Quantitative Cytochemical Technique for Measurement of Glycogen. Nature 195, 504–505 (1962). https://doi.org/10.1038/195504a0
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DOI: https://doi.org/10.1038/195504a0
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