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Histochemical Localization of β-Glucuronidase in Healing Wounds of the Natterjack Toad

Abstract

β-GLUCURONIDASE, the enzyme related to cell proliferation and the formation of connective-tissue ground substance, has been observed in basal layers of the oral mucous membrane and the epidermis, the blood vessels of the normal derma, and healing wounds of the skin of the back, the palate and the tongue in the rat1–3 and healing wounds of the dorsal skin and the tongue in the iguana4. In addition to these experiments I have examined the activity of β-glucuronidase in healing wounds of the dorsal skin and the tongue in an amphibian species, the natterjack toad (Bufo calamita), the animals being killed by decapitation after 4, 7, 14, 21, 28, 35 and 42 days.

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STOLK, A. Histochemical Localization of β-Glucuronidase in Healing Wounds of the Natterjack Toad. Nature 195, 298–299 (1962). https://doi.org/10.1038/195298a0

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