Abstract
C18 OR higher fatty acids can be identified in and extracted from mouse mammary carcinomas and from mouse lymphomatous tissue1–3. Similar substances can be extracted from normal mouse tissue, but they are usually less hæmolytic than are the substances extracted from tumours and lymphomas3. The method used for estimating the quantity of the higher fatty acids is based on their fluorescence in benzene when treated with rhodamine B and uranyl nitrate in benzene. From the apparent amount has to be subtracted the amount of vitamin A and related substances which are soluble in benzene and very fluorescent; this is measured by spectroscopy at 3300 Å.
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References
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PONDER, E., PONDER, R. Extraction of the Higher Fatty Acids from Human Malignant Tumours. Nature 193, 1300–1301 (1962). https://doi.org/10.1038/1931300b0
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DOI: https://doi.org/10.1038/1931300b0
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