Abstract
THE main hydrocarbons present in the essential oil of hop (Humulus lupulus L. var. Fuggles) are myrcene, farnesene, caryophyllene and humulene1 (Fig. 1). In an attempt to find the best conditions for quantitative assay of these substances, hop oil hydrocarbon fraction was examined by gas chromatography over a range of column temperatures. It was noticed that when the temperature of analysis was decreased from 150° C. to 125° C., the separation of caryophyllene and farnesene, incomplete at 150° C., became non-existent at 125° C. However, when the analysis was carried out at 175° C. complete separation was achieved. This is the converse of the expected behaviour of compounds in gas chromatography. The analysis was then carried out at intervals over the range 100°–200° C., and the retention volumes of the constituents determined.
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References
Howard, G. A., and Slater, C. A., Chem. and Indust., 495 (1957).
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ROBERTS, J. Elution Sequence as a Function of Temperature in Gas Chromatography. Nature 193, 1071–1072 (1962). https://doi.org/10.1038/1931071b0
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DOI: https://doi.org/10.1038/1931071b0
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