Movement of Tobacco Mosaic Virus through Epidermis of Nicotiana gluti nosa Leaves

Abstract

IN detached primary cowpea leaves, cucumber mosaic virus requires about 2 hr. at 28° C. to pass through the epidermis to the palisade and to cause local lesions¹. Also, other reports indicate that it takes a certain time for tobacco necrosis virus to move from the infection site on leaves of French bean (Phoseolus vulgaris L. var. Prince) into the palisade layer and that the rate of such movement is greatly influenced by the temperature². Uppal reported that tobacco mosaic virus moved from the upper epidermis of N. sylvestris to the lower epidermis at a rate of 7–8 µ/hr. He was able to detect the virus in the palisade layer about 4 hr. after inoculation of the upper epidermis³.