New Method of preparing Corynebacterium pyogenes Toxoid with High Anti-hæmolysin Titre in Rabbits

Abstract

Corynebacterium pyogenes is generally grown in Robertson's medium¹ when small quantities are prepared and in derivatives of this medium for vaccine production. Producing this medium for vaccines is an expensive time-consuming operation, so it was decided to investigate the possibilities of using fat-free milk as the basic medium. Strains used throughout the work were those recently isolated from bovine lesions and used at present for vaccine production.