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Rapid Preparation of Fluorescent Antibodies using Gel-Filtration

Nature volume 192, pages 182–183 (1961)Cite this article

Abstract

IN the preparation of fluorescent antibodies the excess dye is generally removed by dialysis for 5–6 days (ref. 1). In order to avoid a time-consuming dialysis and to decrease the risk of destroying antibody activity we have used gel-filtration2 in the preparation of a number of fluorescent antibodies, the titres of which were afterwards compared with those of dialysed samples. A preliminary report of the method has already been published3.

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Author information

Authors and Affiliations

  1. Department of Clinical Chemistry,

    JOHAN KILLANDER

  2. Institute of Pathology I,

    JAN PONTÉN

  3. Institute of Medical Chemistry, University of Uppsala,

    LENNART RODÉN

Authors
  1. JOHAN KILLANDER
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  2. JAN PONTÉN
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  3. LENNART RODÉN
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KILLANDER, J., PONTÉN, J. & RODÉN, L. Rapid Preparation of Fluorescent Antibodies using Gel-Filtration. Nature 192, 182–183 (1961). https://doi.org/10.1038/192182b0

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