Abstract
HUMAN and other mammalian red cells have been frozen and thawed with limited haemolysis using a variety of cooling conditions and protective additives. Slow cooling in high concentrations (30 per cent) of glycerol1, moderately rapid cooling in plasma containing sugars2, and ultra-rapid cooling in low concentrations (6 per cent) of glucose3 have allowed high recoveries (90–95 per cent) of red cells of which 80–90 per cent survive at least 24 hr. when transfused.
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Smith, A. V., Lancet, ii, 910 (1950). Sloviter, H. A., ibid., i, 1350 (1950). Mollison, P. L., and Sloviter, H. A., ibid., ii, 862 (1951).
Florio, L., Stewart, P. H. M., and Mugrage, E. R., J. Lab. Clin. Med., 28, 1486 (1943). Strumia, M. M., Colwell, L. C., and Strumia, P. V., Science, 128, 1002 (1958).
Luyet, B. J., Biodynamica, 6, 217 (1949). Meryman, H. T., and Kafig, E., Proc. Soc. Exp. Biol. Med., 90, 587 (1955).
Bricka, M., and Bessis, M., C.R. Soc. Biol., Paris, 149, 875 (1955). Glauser, S. C., and Talbot, T. R., Amer. J. Med. Sci., 231, 75 (1956). Steinbuch, M., and Quentin, M., Vox Sang., 3, 123 (1958).
Doebbler, G. F., and Rinfret, A. P., Fed. Proc., 18, 564 (1959); 19, 67 (1960).
Sloviter, H. A., Fed. Proc., 17, 313 (1958).
Sterling, K., and Cray, S. J., J. Clin. Invest., 29, 1614 (1950).
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DOEBBLER, G., BUCHHEIT, R. & RINFRET, A. Recovery and in vivo Survival of Rabbit Erythrocytes. Nature 191, 1405 (1961). https://doi.org/10.1038/1911405a0
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DOI: https://doi.org/10.1038/1911405a0
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