Abstract
THE combination of two different proteins with each other via covalent bonds is useful for certain immunological work1–4. This type of chemical reaction requires a bifunctional compound which, by stepwise coupling first with one and then with the other protein molecule, will form a bridge between them. Among the compounds used for this purpose have been bis-diazotized benzidine1 and difluoro-dinitrobenzene2. Howard and Wild3 developed a method consisting of three steps: one protein was coupled with bis-diazotized benzidine at acid pH, the second protein was coupled with a derivative of pyrrole at basic pH, and then the products of both additions were coupled with each other.
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References
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BOREK, F. A New Two-Stage Method for Cross-linking Proteins. Nature 191, 1293–1294 (1961). https://doi.org/10.1038/1911293a0
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DOI: https://doi.org/10.1038/1911293a0
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