Abstract
IN an attempt to study the plasma membrane of cells by correlation of physico-chemical experiments and electron microscopical observations, yeast protoplasts were considered as a suitable experimental material. These protoplasts are easily obtained in quantities sufficient for chemical experiments by treatment of a yeast cell suspension with gut juice of Helix pomatia1–3. In the present work Saccharomyces carlsbergensis strain 74 of the National Collection of Yeast Cultures, Britain, has been used.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Giaja, J., C.R. Soc. Biol., 82, 719 (1919).
Eddy, A. A., and Williamson, D. H., Nature, 179, 1252 (1957).
Eddy, A. A., and Williamson, D. H., Nature, 183, 1101 (1959).
Thorsson, K. G., and Weibull, C., J. Ultrastr. Res., 1, 412 (1958).
Lee, S., Exp. Cell Res., 21, 252 (1960).
Eddy, A. A., Exp. Cell Res., 17, 447 (1959).
Cook, A. H., The Chemistry and Biology of Yeast (Academic Press, New York, 1958).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
ELBERS, P. Fixation of Yeast Protoplasts for Electron Microscopy. Nature 191, 1022–1023 (1961). https://doi.org/10.1038/1911022a0
Issue Date:
DOI: https://doi.org/10.1038/1911022a0
This article is cited by
-
Controlled production of spheroplasts in the yeast Nadsonia elongata
Archiv f�r Mikrobiologie (1973)
-
Formation of protoplasts ofFusarium culmorum by strepzyme
Antonie van Leeuwenhoek (1964)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
