Abstract
IT has previously been shown that the blood and urine of rabbits suffering from hæmolytic anæmia contain a mucoprotein humoral factor, hæmopoietine or erythropoietine, which on injection into normal animals stimulates erythropoiesis1. The present experiments were undertaken to find out what effect this factor might have on nucleic acid biosynthesis in normal rabbit tissues in vitro. A crude preparation of erythropoietine was obtained from the urine of animals made anæmic with phenylhydrazine. The urine was brought to pH 4.5 with hydrochloric acid, and 4 volumes of ethanol were added. After standing overnight at 4° C. the precipitate was centrifuged down, washed with ethanol and ether, and dried. The dry powder was extracted with a small volume of Krebs–Ringer bicarbonate buffer. The protein concentration of this ‘anæmic urine extract’ was determined by the biuret method. A ‘normal urine extract’ was similarly prepared from the urine of normal rabbits. The effects of both extracts on the incorporation of formate labelled with carbon-14 into the nucleic acids of bone marrow, spleen and liver in vitro were determined by the methods of Smellie et al. 2.
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Hodgson, G., Perretta, M., Yudilevich, D., and Eskuche, I., Proc. Soc. Exp. Biol. Med., 99, 137 (1958).
Smellie, R. M. S., Thomson, R. Y., and Davidson, J. N., Biochim. Biophys. Acta, 29, 59 (1958).
Thomson, R. Y., Perretta, M. A., and Ricceri, G., Biochim. Biophys. Acta, 45, 87 (1960).
Smellie, R. M. S., Humphrey, G. F., Kay, E. R. M., and Davidson, J. N., Biochem. J., 60, 177 (1955).
Lajtha, L. G., and Vane, J. R., Nature, 182, 191 (1958).
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PERRETTA, M., THOMSON, R. Effect of Erythropoietine on Incorporation of Formate labelled with Carbon-14 into the Nucleic Acids of Normal Rabbit Tissues in vitro . Nature 190, 912 (1961). https://doi.org/10.1038/190912a0
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DOI: https://doi.org/10.1038/190912a0
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