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Method for revealing the Membrane Systems in Micro-organisms

Abstract

THE use of potassium permanganate as a biological fixative for revealing the membrane systems of cells is well known to electron microscopists1. This fixative has been successful in preserving the fine morphology of bacteria and yeast; but, up to date, workers have failed to show the true nature of the membranes in these organisms2,3. This communication describes a method for stabilizing and increasing the electron density of membranes in micro-organisms by post-fixiiig them with a uranium salt.

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References

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  2. Hashimoto, T., Gerhardt, P., Conti, S. F., and Naylor, H. B., J. Biophys. and Biochem. Cytol., 7, 305 (1960).

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NORTH, R. Method for revealing the Membrane Systems in Micro-organisms. Nature 190, 1215–1216 (1961). https://doi.org/10.1038/1901215a0

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  • DOI: https://doi.org/10.1038/1901215a0

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