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Examination of Leptospira pomona by Gel Diffusion

Abstract

IN recent years, diffusible antigens of many bacteria and viruses have been demonstrated by the double-diffusion precipitation reaction in agar. Except for the work of Rothstein and Hiatt1, who used the Oudin2 technique in the study of chemically prepared fractions, we do not know of any investigation involving application of these methods in the study of Leptospira. This communication describes the results of an antigenic analysis of the four sub-serotypes of Leptospira pomona, attempted by diffusion of antigens and antibodies in agar.

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PANDE, P., SEKARIAH, P. & SHUKLA, R. Examination of Leptospira pomona by Gel Diffusion. Nature 189, 511–512 (1961). https://doi.org/10.1038/189511a0

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