Abstract
IN the course of investigations of the movement of nutrient ions through barley roots, it became necessary to stop the movement instantaneously and study the distribution of the nutrient ions at that time. A technique was devised that involved stopping the reaction at liquid-nitrogen temperature and sectioning the frozen tissue for subsequent radioautography. The purpose of this communication is to report on a method of preparation of fresh frozen sections involving no prior fixation of the tissue.
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FRIED, M., FRANKLIN, A. Rapid Preparation of Fresh Frozen Sections of Barley Roots. Nature 189, 414–415 (1961). https://doi.org/10.1038/189414a0
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DOI: https://doi.org/10.1038/189414a0
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