Abstract
THE present communication reports changes produced by ultra-violet irradiation in thymidylic acid (A), deoxycytidylic acid (B), apurinic acid (C), deoxyribonucleic acid (D) and a 1 : 1 mixture of A and B, and also the reversibility of these changes in C and D. The conditions of the irradiation are described in a previous paper1. Apurinic acid was prepared according to the method of Tamm, Hoder and Chargaff2. The concentration of the solutions was such that at pH 7 (phosphate buffer) the optical density readings at the maxima were 1.000 ± 2 per cent. The irradiation time was 20 min. for each solution, and the decrease of optical density at 260 mµ was recorded at 5 min. intervals, as shown in Table 1.
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References
Wang, S. Y., J. Amer. Chem. Soc., 80, 6196 (1958).
Tamm, C., Hoder, M. E., and Chargaff, E., J. Biol. Chem., 195, 49 (1952).
Wang, S. Y., Nature, 184, B.A. 59 (1959).
Kland, M. J., and Johnson, L. A., J. Amer. Chem. Soc., 79, 6197 (1957).
Beukers, R., Ijlstra, J., and Berends, W., Rec. Trav. Chim., 78, 247 (1959), has reported an observation similar to ours.
Wang, S. Y., Nature, 184, 184 (1959).
Shugar, D., and Wierzchowski, K. L., J. Polymer Sci., 31, 269 (1958).
Forbes, G. S., and Heidt, L. J., J. Amer. Chem. Soc., 56, 2363 (1934).
Beukers, R., Ijlstra, J., and Berends, W., Rec. Trav. Chim., 78, 101 (1960).
Beukers, R., Ijlstra, J., and Berends, W., Rec. Trav. Chim., 77, 729 (1958).
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WANG, S. Reversible Behaviour of the Ultra-violet Irradiated Deoxyribonucleic Acid and its Apurinic Acid. Nature 188, 844–846 (1960). https://doi.org/10.1038/188844a0
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DOI: https://doi.org/10.1038/188844a0
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