Abstract
AS pointed out by Dr. J. G. Feinberg, the pattern of wells in the agar-gel plate (Fig. 2) of our communication in Nature of October 24, 1959 1, lends itself to misinterpretation. However, the presence of common antigenic determinants in extracts of T. rubrum and A. fumigatus, which figure in the plate, was demonstrated by inhibition tests. These, together with all the other tests in our communication, were performed on conventional agar-gel patterns (Fig. 1, ref. 1). Nine sera were tested for their capacity to precipitate against these two fungal extracts, after the addition of each of them to the serum. The addition of A. fumigatus extract resulted on one hand in complete inhibition of its own precipitation lines with six and partial inhibition with the remaining three sera, and on the other hand in complete inhibition of the T. rubrum extract precipitation lines with seven and partial inhibition with a further one serum. The addition of T. rubrum extract resulted in complete inhibition of the precipitation lines of the A. fumigatus extract with six sera, and of its own precipitation lines with all nine sera.
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References
Pepys, J., Riddell, R. W., and Clayton, Y. M., Nature, 184, 1328 (1959).
Pepys, J., Augustin, R., and Paterson, A. B., Tubercle, Lond., 40, 163 (1959).
Pepys, J., Riddell, R. W., Citron, K. M., Short, E. I., and Clayton, Y. M., Amer. Rev. Respir. Dis., 80, 167 (1959).
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PEPYS, J., RIDDELL, R. & CLAYTON, Y. Misinterpretation of an Agar-Gel Precipitation Pattern. Nature 188, 685 (1960). https://doi.org/10.1038/188685a0
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DOI: https://doi.org/10.1038/188685a0
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