Abstract
SAMPLES of human plasminogen, prepared according to the method of Kline1, have been subjected to ultracentrifugal analysis2. The patterns observed contained two sedimenting peaks. The slower peak constituted approximately 70 per cent of the total area and had a sedimentation constant, s 20,w, of 4.28 S, extrapolated to infinite dilution. The faster peak, which was much broader and resolved poorly from the main component, had a sedimentation constant of about 7–10 S. It was merely assumed that plasminogen was to be identified with the 4.3 S-component, and on this basis, along with other data, molecular size and shape were estimated. We now report direct evidence to substantiate this assumption.
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References
Kline, D. L., J. Biol. Chem., 204, 949 (1953).
Shulman, S., Alkjaersig, N., and Sherry, S., J. Biol. Chem., 233, 91 (1958).
Remmert, L. F., and Cohen, P. P., J. Biol. Chem., 181, 431 (1949).
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SHULMAN, S. Identification of Ultracentrifugal Components in Human Plasminogen Preparations. Nature 188, 161–162 (1960). https://doi.org/10.1038/188161b0
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DOI: https://doi.org/10.1038/188161b0
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