Abstract
IN the course of investigating various methods of isolating skin-sensitizing antibodies from human allergic serum, a procedure has been devised which could be applied to the rapid preparation of other 7S-type gamma-globulins in a high degree of purity. The technique employs diethyl amino ethyl cellulose anion exchanger, prepared according to the method used by Peterson and Sober1, to adsorb out of the serum all proteins other than 7S-type gamma-globulins. For this purpose, a batch process has proved superior to the more widely used column chromatography, because of its greater speed and ease of manipulation.
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References
Peterson, E. A., and Sober, H. A., J. Amer. Chem. soc., 28, 751 (1956).
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STANWORTH, D. A Rapid Method of preparing Pure Serum Gamma-Globulin. Nature 188, 156–157 (1960). https://doi.org/10.1038/188156a0
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DOI: https://doi.org/10.1038/188156a0
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