Abstract
ADRENALECTOMY enhances, and adrenal cortical hormone depresses, the incorporation of carbon-14 from amino-acids, and from amino-acid precursors, into the protein of isolated rat diaphragm1,2. These changes are, at least in part, the result of a direct effect on protein biosynthesis1,3. Whether corticosteroids also effect protein synthesis indirectly by influencing amino-acid transport and accumulation is not known. The possibility was tested by measuring the penetration and accumulation of [1-14C-]-aminoisobutyric acid4, a non-utilizable amino-acid analogue, and of the utilizable amino-acids [U-14C] L-phenylalanine and [U-14C] L-lysine, by ‘intact’ diaphragms5 isolated from normal, adrenalectomized, and cortisone-treated rats.
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WOOL, I. Accumulation of Substrate by Isolated Rat Diaphragm: a Possible Mechanism for the Anti-Inflammatory Action of Corticosteroids. Nature 186, 728 (1960). https://doi.org/10.1038/186728a0
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DOI: https://doi.org/10.1038/186728a0
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