Abstract
SINCE July 1959 we have been recording spontaneous potentials from explants of brain in tissue culture. The tissue used was cerebellum from chick embryos of 8 days incubation and older. The supernatant was a 0.25 per cent solution of human serum protein in a physiologically balanced salt solution. The composition of the physiological balanced salt-solution is as follows : 
The other techniques were those described in publications from this laboratory1–3.
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References
Cunningham and Estborn, Lab. Invest., 7, 156 (1958).
Estborn, Cunningham, et al., Lab. Invest., 7, 524 (1958).
Cunningham and Herbst, Lab. Invest. (in the press).
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CUNNINGHAM, A., DOUGHERTY, M. & RYLANDER, B. Spontaneous Potentials from Explants of Brain Tissue in Culture. Nature 186, 477–478 (1960). https://doi.org/10.1038/186477a0
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DOI: https://doi.org/10.1038/186477a0
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